Tg(Nphs1-IL2RA)18Mska
Transgene Detail

Summary

• Symbol: Tg(Nphs1-IL2RA)18Mska
• Name: transgene insertion 18, Taiji Matsusaka
• MGI ID: MGI:3762500
• Synonyms: NEP25, Nphs1-CD25
• Transgene: Tg(Nphs1-IL2RA)18Mska Location: unknown
• Alliance: Tg(Nphs1-IL2RA)18Mska page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1 x C57BL/6N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Nphs1-IL2RA)18Mska expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IL2RA (3559)

  Il2ra

  (MGI:96549)

• Mutation details: A 5527 bp fragment of the mouse Nphs1 gene was used as the transgene promoter. Two ATG sequences in the first intron of the fragment were disrupted by site-directed mutagenesis to prevent initiation of transcription of Nphs1. A fragment containing the human IL2RA (CD25) cDNA, FLAG sequence, and polyadenylation signal of the human growth hormone gene were placed downstream of the Nphs1 fragment in a transgenic construct. The vector was injected into (C57BL/6 x DBA/2) x C57BL/6N fertilized eggs. Two lines 9 and 18 showed essentially the same phenotype, and line 18 was maintained by backcrossing with C57BL/6N mice. Immunohistochemistry demonstrated that the transgene is expressed in podocytes, and RT-PCR showed transgene expression in the brain and pancreas but not in other organs (heart, spleen, thymus, lung, or muscle). (J:127013)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:127013 Matsusaka T, et al., Genetic engineering of glomerular sclerosis in the mouse via control of onset and severity of podocyte-specific injury. J Am Soc Nephrol. 2005 Apr;16(4):1013-23
• All: 16 reference(s)