Zpbp2^tm1Zuk
Targeted Allele Detail

Summary

• Symbol: Zpbp2^tm1Zuk
• Name: zona pellucida binding protein 2; targeted mutation 1, Martin M Matzuk
• MGI ID: MGI:3762590
• Gene: Zpbp2 Location: Chr11:98441923-98449491 bp, + strand Genetic Position: Chr11, 61.97 cM, cytoband D
• Alliance: Zpbp2^tm1Zuk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126661
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1, 2 and 3 were replaced with a PgkHPRT cassette via homologous recombination. This resulted in deletion of the signal peptide encoding region. Northern and Western blot analysis confirmed the absence of RNA transcript and protein in the testes. (J:126661)

Expression

In Mice Carrying this Mutation:	18 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zpbp2 Mutation: 25 strains or lines available

References

• Original: J:126661 Lin YN, et al., Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis. Mol Cell Biol. 2007 Oct;27(19):6794-805
• All: 4 reference(s)