Gatad2a^tm1Rnu
Targeted Allele Detail

Summary

• Symbol: Gatad2a^tm1Rnu
• Name: GATA zinc finger domain containing 2A; targeted mutation 1, Roel Nusse
• MGI ID: MGI:3762633
• Synonyms: mp66alpha null, p66 KO
• Gene: Gatad2a Location: Chr8:70359726-70449034 bp, - strand Genetic Position: Chr8, 34.06 cM
• Alliance: Gatad2a^tm1Rnu page

Gatad2a^tm1Rnu/Gatad2a^tm1Rnu mutant embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126871
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 2-4 with a loxP-flanked neomycin resistance (pgk-neo) cassette. This mutation introduced a stop codon before the coiled-coil region (CR1) region of the gene. In order to remove the floxed pgk-neo cassette, heterozygous mutant females were crossed to a CMV-cre male on a mixed C57BL/6-129SV background. (J:126871)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gatad2a Mutation: 126 strains or lines available

References

• Original: J:126871 Marino S, et al., Mutants in the mouse NuRD/Mi2 component P66alpha are embryonic lethal. PLoS One. 2007;2(6):e519
• All: 1 reference(s)