Git2^Gt(XG510)Byg
Gene trapped Allele Detail

Summary

• Symbol: Git2^Gt(XG510)Byg
• Name: GIT ArfGAP 2; gene trap XG510, BayGenomics
• MGI ID: MGI:3762635
• Synonyms: GIT2^-
• Gene: Git2 Location: Chr5:114865469-114912647 bp, - strand Genetic Position: Chr5, 55.99 cM
• Alliance: Git2^Gt(XG510)Byg page

Mutation origin

• Mutant Cell Line: XG510
• Germline Transmission: Earliest citation of germline transmission: J:126878
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap vetor containing a beta-geo cassette was inserted into intron 2. Insertion was confirmed by PCR genotyping and expression of the lacZ gene was confirmed by beta-galactosidase staining. Two transcripts were detected by RT-PCR: one the expected fusion of exon 1 and 2 to the gene trap vector sequence and the other an alternative transcript lacking exon 2. (J:126878)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XG510	CC183093 ; (MGI Seq Detail)	5' RACE	Chr5:114911377-114911520 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  12 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Git2 Mutation: 78 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:126878 Schmalzigaug R, et al., Differential expression of the ARF GAP genes GIT1 and GIT2 in mouse tissues. J Histochem Cytochem. 2007 Oct;55(10):1039-48
• All: 14 reference(s)