St8sia4^tm1.1Mifu
Targeted Allele Detail

Summary

• Symbol: St8sia4^tm1.1Mifu
• Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4; targeted mutation 1.1, Minoru Fukuda
• MGI ID: MGI:3762640
• Synonyms: ST8IV^-
• Gene: St8sia4 Location: Chr1:95515407-95595296 bp, - strand Genetic Position: Chr1, 47.76 cM, cytoband D
• Alliance: St8sia4^tm1.1Mifu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126687
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A floxed neo cassette was inserted into the 5' untranslated region and an additional loxP site was inserted downstream of exon 1. Cre-mediated recombination by transient expression of cre deleted the exon 1. which contains the initiation methionine and a transmembrane domain. Without the signal peptide and the transmembrane domain no functional protein is made. The lack of transcript was confirmed by RT-PCR. (J:126687)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any St8sia4 Mutation: 28 strains or lines available

References

• Original: J:126687 Angata K, et al., Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development. Mol Cell Biol. 2007 Oct;27(19):6659-68
• All: 2 reference(s)