Kiss1^tm1Rla
Targeted Allele Detail

Summary

• Symbol: Kiss1^tm1Rla
• Name: KiSS-1 metastasis-suppressor; targeted mutation 1, Risto Lapatto
• MGI ID: MGI:3762755
• Gene: Kiss1 Location: Chr1:133254950-133257460 bp, + strand Genetic Position: Chr1, 57.91 cM
• Alliance: Kiss1^tm1Rla page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126704
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced with a neomycin resistance cassette. Southern blotting confirmed recombination but RT-PCR of exon 2 detected a transcript from the gene. Amplification and sequencing of the transcript revealed a previously unknown exon upstream of the targeted exon that was splicing to exon 2. Because no translational start site was found in this sequence, the mutation was predicted not to produce a functional protein. This was confirmed by immunohistochemistry on the hypothalamus. (J:126704)

Disease models

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kiss1 Mutation: 22 strains or lines available

References

• Original: J:126704 Lapatto R, et al., Kiss1-/- mice exhibit more variable hypogonadism than Gpr54-/- mice. Endocrinology. 2007 Oct;148(10):4927-36
• All: 7 reference(s)