Cd274^tm1.1Lex
Targeted Allele Detail

Summary

• Symbol: Cd274^tm1.1Lex
• Name: CD274 antigen; targeted mutation 1.1, Lexicon Genetics
• MGI ID: MGI:3762770
• Synonyms: PD-L1^-
• Gene: Cd274 Location: Chr19:29344855-29365495 bp, + strand Genetic Position: Chr19, 23.88 cM, cytoband C2
• Alliance: Cd274^tm1.1Lex page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126829
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: By homologous recombination a loxP site was inserted 5' of exon2, followed by a NeoR cassette and a 3' loxP site. The resulting mutant mice, Cd274^tm1Lex, were crossed with protamine-Cre transgenic mice to delete exon 2 containing the translational start site. Flow cytometric protein expression analysis confirmed absence of targeted protein expression. (J:126829)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd274 Mutation: 70 strains or lines available

References

• Original: J:126829 Carter LL, et al., PD-1/PD-L1, but not PD-1/PD-L2, interactions regulate the severity of experimental autoimmune encephalomyelitis. J Neuroimmunol. 2007 Jan;182(1-2):124-34
• All: 1 reference(s)