Prnp^{tm2(PRNP*129M)Tkit}
Targeted Allele Detail

Summary

• Symbol: Prnp^{tm2(PRNP*129M)Tkit}
• Name: prion protein; targeted mutation 2, Tetsuyuki Kitamoto
• MGI ID: MGI:3762784
• Synonyms: Ki-Hu129M
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp^{tm2(PRNP*129M)Tkit} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:105882
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Prnp^{tm2(PRNP*129M)Tkit} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  PRNP (5621)

  Prnp	(MGI:97769)

• Mutation details: The open reading frame was replaced with the human PRNP variant with a methionine at amino acid position 129 such that the mouse signal sequence was retained. Expression was confirmed by western blot analysis. (J:105882)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 142 strains or lines available

References

• Original: J:105882 Asano M, et al., vCJD prion acquires altered virulence through trans-species infection. Biochem Biophys Res Commun. 2006 Mar 31;342(1):293-9
• All: 4 reference(s)