Irak4^tm2Aki
Targeted Allele Detail

Summary

• Symbol: Irak4^tm2Aki
• Name: interleukin-1 receptor-associated kinase 4; targeted mutation 1, Shizou Akira
• MGI ID: MGI:3762878
• Synonyms: IRAK-4 (KK213AA), IRAK-4^KN
• Gene: Irak4 Location: Chr15:94441495-94466198 bp, + strand Genetic Position: Chr15, 48.55 cM, cytoband F1
• Alliance: Irak4^tm2Aki page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125729
• Parent Cell Line: GSI-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A targeting vector was constructed so that serines at positions 213-214 were replaced by alanines (codons "CTTCTT" were mutated to "CGCCGC")(SS213-215AA). This mutation occurs in the ATP-binding pocket of the protein and disrupts its kinase activity. A floxed neomycin cassette was also included. F1 mice produced from chimeras were crossed with Cre-transgenic mice to excise the neomycin cassette. Western analysis on macrophages from homozygote null mice indicated the mutant protein was expressed at slightly reduced levels. Abolition of kinase activity of the protein was confirmed by an in vitro assay. (J:125729)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Irak4 Mutation: 32 strains or lines available

References

• Original: J:125729 Kawagoe T, et al., Essential role of IRAK-4 protein and its kinase activity in Toll-like receptor-mediated immune responses but not in TCR signaling. J Exp Med. 2007 May 14;204(5):1013-24
• All: 1 reference(s)