Irak4^tm1Lix
Targeted Allele Detail

Summary

• Symbol: Irak4^tm1Lix
• Name: interleukin-1 receptor-associated kinase 4; targeted mutation 1, Xiaoxia Li
• MGI ID: MGI:3762911
• Synonyms: IRAK4^KDKI, IRAK4 KI
• Gene: Irak4 Location: Chr15:94441495-94466198 bp, + strand Genetic Position: Chr15, 48.55 cM, cytoband F1
• Alliance: Irak4^tm1Lix page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125732
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting vector was constructed where lysines 213 and 214 that are part of the ATP-binding pocket of the protein were mutated to methionines (KK213-214MM). An FRT-flanked neomycin selection cassette was also included and then subsequently removed leaving behind a single FRT site. Western blots confirmed the mutant protein has similar expression to wild-type protein. Abolition of kinase activity of the protein was confirmed by an in vitro assay. (J:125732)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Irak4 Mutation: 32 strains or lines available

References

• Original: J:125732 Kim TW, et al., A critical role for IRAK4 kinase activity in Toll-like receptor-mediated innate immunity. J Exp Med. 2007 May 14;204(5):1025-36
• All: 12 reference(s)