Gria1^tm4Rlh
Targeted Allele Detail

Summary

• Symbol: Gria1^tm4Rlh
• Name: glutamate receptor, ionotropic, AMPA1 (alpha 1); targeted mutation 4, Richard Huganir
• MGI ID: MGI:3762912
• Gene: Gria1 Location: Chr11:56902342-57221070 bp, + strand Genetic Position: Chr11, 34.51 cM
• Alliance: Gria1^tm4Rlh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126784
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Serines 831, 839, 845 and threonines 838 and 840 were mutated to alanine by PCR mutagenesis prior to homologous recombination. A floxed neo cassette was inserted upstream of the last conding exon was removed by cre-mediated recombination following a cross to mice containing Tg(CMV-cre)1Nagy. Western blot analysis with was performed to confirm the presence of the protein but loss of the phosphorylation sites. (J:126784)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gria1 Mutation: 65 strains or lines available

References

• Original: J:126784 Lee HK, et al., Identification and characterization of a novel phosphorylation site on the GluR1 subunit of AMPA receptors. Mol Cell Neurosci. 2007 Sep;36(1):86-94
• All: 3 reference(s)