Mir155^tm1.1Rsky
Targeted Allele Detail

Summary

• Symbol: Mir155^tm1.1Rsky
• Name: microRNA 155; targeted mutation 1.1, Klaus Rajewsky
• MGI ID: MGI:3763386
• Synonyms: bic/miR-155-, miR-155^KO
• Gene: Mir155 Location: Chr16:84511028-84511092 bp, + strand Genetic Position: Chr16, 46.92 cM
• Alliance: Mir155^tm1.1Rsky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:121084
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A modified bacterial artificial chromosome (BAC) targeting vector was used to replace a 0.97 kb portion of exon 2 of the bic/mir-155 gene with an in-frame b-galactosidase (lacZ) reporter gene (with polyA sequence and followed by a loxP-flanked neomycin resistance cassette). The mice were bred with a cre-deleter strain (congenic C57BL/6 background) to remove the loxP-flanked selection cassette. The resulting bic/mir-155 mutant mice (with lacZ followed by a single remaining loxP site). In heterozygotes, approximately 60% of germinal center (GC) B cells express the lacZ reporter whereas the vast majority of the non-GC B cells do not. (J:121084)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mir155 Mutation: 13 strains or lines available

References

• Original: J:121084 Thai TH, et al., Regulation of the germinal center response by microRNA-155. Science. 2007 Apr 27;316(5824):604-8
• All: 117 reference(s)