Mir150^tm1Rsky
Targeted Allele Detail

Summary

• Symbol: Mir150^tm1Rsky
• Name: microRNA 150; targeted mutation 1, Klaus Rajewsky
• MGI ID: MGI:3763431
• Synonyms: MiR-150
• Gene: Mir150 Location: Chr7:44771181-44771245 bp, + strand Genetic Position: Chr7, 29.14 cM
• Alliance: Mir150^tm1Rsky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127234
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert an frt-flanked pGK-Neo cassette 468 bp upstream of the miR-150 stem loop, with one loxP site placed immediately upstream of the pGK-Neo cassette and the other loxP site placed 146 bp downstream of the miR-150 stem loop. To delete the loxP site-flanked 679 bp miR-150 coding genomic region and the frt-flanked pGK-Neo cassette, mice were then bred to cre-expressing mice (C57BL/6 congenic background, similar to Stock No. 006054). The resulting mice have a single loxP site replacing the miR-150 coding region. (J:127234)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mir150 Mutation: 6 strains or lines available

References

• Original: J:127234 Xiao C, et al., MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell. 2007 Oct 5;131(1):146-59
• All: 22 reference(s)