Mdm2^tm1Ypz
Targeted Allele Detail

Summary

• Symbol: Mdm2^tm1Ypz
• Name: transformed mouse 3T3 cell double minute 2; targeted mutation 1, Yanping Zhang
• MGI ID: MGI:3763432
• Synonyms: Mdm2^C462A
• Gene: Mdm2 Location: Chr10:117524780-117546663 bp, - strand Genetic Position: Chr10, 66.32 cM, cytoband C1-C3
• Alliance: Mdm2^tm1Ypz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126004
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Nucleotide substitutions
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted into intron 10 and cystine codon 462 (TGT) in exon 12 was changed to alanine (GCC) (p.C462A). This amino acid substitution abolishes E3 ubiquitin ligase activity without affecting DNA binding ability. The neo cassette was subsequently removed by Cre-mediated recombination. (J:126004)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mdm2 Mutation: 54 strains or lines available

References

• Original: J:126004 Itahana K, et al., Targeted Inactivation of Mdm2 RING finger E3 ubiquitin ligase activity in the mouse reveals mechanistic insights into p53 regulation. Cancer Cell. 2007 Oct;12(4):355-66
• All: 5 reference(s)