Dnmt1tm2Jrc
Targeted Allele Detail
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Symbol: |
Dnmt1tm2Jrc |
Name: |
DNA methyltransferase 1; targeted mutation 2, J Richard Chaillet |
MGI ID: |
MGI:3763529 |
Synonyms: |
Dnmt1V |
Gene: |
Dnmt1 Location: Chr9:20818501-20871084 bp, - strand Genetic Position: Chr9, 7.66 cM
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Alliance: |
Dnmt1tm2Jrc page
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Allele Type: |
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Targeted |
Mutation: |
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Insertion
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Mutation details: Three point mutations were introduced changing the initiation codon in exon 1s into an EcoRV site. The Pgk1-neo selection cassette was removed by transient Cre-mediated recombination in the ES cells. The point mutations resulted in transcripts initiated from exon 1s being translated into the splice form Dnmt1o rather than Dnmt1. In spleens from heterozygous mice the wild-type and mutant transcripts are expressed at equal levels. However, methyltransferase activity in the spleen is increased about 2.7- and 4.5-fold (compared to wild-type mice) in heterozygous and homozygous mice, respectively.
(J:126389)
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Dnmt1 Mutation: |
112 strains or lines available
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Original: |
J:126389 Ding F, et al., In vivo stabilization of the Dnmt1 (cytosine-5)- methyltransferase protein. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14861-6 |
All: |
4 reference(s) |
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