Dnmt1^tm2Jrc
Targeted Allele Detail

Summary

• Symbol: Dnmt1^tm2Jrc
• Name: DNA methyltransferase 1; targeted mutation 2, J Richard Chaillet
• MGI ID: MGI:3763529
• Synonyms: Dnmt1^V
• Gene: Dnmt1 Location: Chr9:20818501-20871084 bp, - strand Genetic Position: Chr9, 7.66 cM
• Alliance: Dnmt1^tm2Jrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126389
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Three point mutations were introduced changing the initiation codon in exon 1s into an EcoRV site. The Pgk1-neo selection cassette was removed by transient Cre-mediated recombination in the ES cells. The point mutations resulted in transcripts initiated from exon 1s being translated into the splice form Dnmt1o rather than Dnmt1. In spleens from heterozygous mice the wild-type and mutant transcripts are expressed at equal levels. However, methyltransferase activity in the spleen is increased about 2.7- and 4.5-fold (compared to wild-type mice) in heterozygous and homozygous mice, respectively. (J:126389)

Expression

In Mice Carrying this Mutation:

14 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dnmt1 Mutation: 112 strains or lines available

References

• Original: J:126389 Ding F, et al., In vivo stabilization of the Dnmt1 (cytosine-5)- methyltransferase protein. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14861-6
• All: 4 reference(s)