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Dnmt1tm2Jrc
Targeted Allele Detail
Summary
Symbol: Dnmt1tm2Jrc
Name: DNA methyltransferase 1; targeted mutation 2, J Richard Chaillet
MGI ID: MGI:3763529
Synonyms: Dnmt1V
Gene: Dnmt1  Location: Chr9:20818501-20871084 bp, - strand  Genetic Position: Chr9, 7.66 cM
Alliance: Dnmt1tm2Jrc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:126389
Parent Cell Line:  W9.5/W95 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion
 
Mutation detailsThree point mutations were introduced changing the initiation codon in exon 1s into an EcoRV site. The Pgk1-neo selection cassette was removed by transient Cre-mediated recombination in the ES cells. The point mutations resulted in transcripts initiated from exon 1s being translated into the splice form Dnmt1o rather than Dnmt1. In spleens from heterozygous mice the wild-type and mutant transcripts are expressed at equal levels. However, methyltransferase activity in the spleen is increased about 2.7- and 4.5-fold (compared to wild-type mice) in heterozygous and homozygous mice, respectively. (J:126389)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 14 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Dnmt1 Mutation:  112 strains or lines available
References
Original:  J:126389 Ding F, et al., In vivo stabilization of the Dnmt1 (cytosine-5)- methyltransferase protein. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14861-6
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory