Egfr^tm1Msi
Targeted Allele Detail

Summary

• Symbol: Egfr^tm1Msi
• Name: epidermal growth factor receptor; targeted mutation 1, Maria Sibilia
• MGI ID: MGI:3763534
• Synonyms: EGFR^f
• Gene: Egfr Location: Chr11:16702203-16868158 bp, + strand Genetic Position: Chr11, 9.41 cM, cytoband A1-A4
• Alliance: Egfr^tm1Msi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125912
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector in which the promoter and the first exon of the gene are flanked by loxP sites was constructed. The upstream loxP site with a diagnostic XmaCI restriction site was placed into a BSMI site, and the downstream loxP site was cloned into the SexA1 site. A frt-flanked neomycin cassette was placed between exon 1 and the downstream loxP site. Recombination was confirmed through PCR and Southern blot analysis. (J:125912)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Egfr Mutation: 87 strains or lines available

References

• Original: J:125912 Natarajan A, et al., The EGF receptor is required for efficient liver regeneration. Proc Natl Acad Sci U S A. 2007 Oct 23;104(43):17081-6
• All: 25 reference(s)