Gt(ROSA)26Sor^{tm2(Mirn150)Rsky}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(Mirn150)Rsky}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Klaus Rajewsky
• MGI ID: MGI:3763536
• Synonyms: Gt(ROSA)26Sor^{tm2(Mir150)Rsky}, mir150-EGFP Tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(Mirn150)Rsky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127234
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(Mirn150)Rsky} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mir150 (MGI:2676835)

• Mutation details: Genomic DNA (including the Mirn150 stem loop with 136 bp of 5' and 130 bp of 3' flanking sequence) preceded by the CAG promoter and a loxP-flanked neo-STOP cassette was targeted to the ROSA26 locus. An frt-flanked IRES-EGFP cassette was positioned between the Mirn150 genomic DNA and the polyadenylation signal. The targeting construct was injected into R19 ES cells that were injected into tetraploid blastocysts. Removal of the floxed neo-STOP cassette via cre-mediated excision (by breeding to Tg(CMV-cre)1Cgn mice) resulted in CAG-driven production of Mirn150 and EGFP in all tissues. This allele shares the same progenitor as allele Gt(ROSA)26Sor^{tm14.1(Mirn150)Rsky} and is the progenitor allele for Gt(ROSA)26Sor^{tm2.1(Mirn150)Rsky}. (J:127234)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

ES cell line = R19 (ARF1 R19 ES cells from Artemis Pharmaceuticals, Germany)

References

• Original: J:127234 Xiao C, et al., MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell. 2007 Oct 5;131(1):146-59
• All: 1 reference(s)