Gt(ROSA)26Sor^{tm2.1(Mirn150)Rsky}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(Mirn150)Rsky}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Klaus Rajewsky
• MGI ID: MGI:3763537
• Synonyms: Gt(ROSA)26Sor^{tm2.1(Mir150)Rsky}, mir150 Tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(Mirn150)Rsky} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127234
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2.1(Mirn150)Rsky} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mir150 (MGI:2676835)

• Mutation details: Gt(ROSA)26Sor^{tm2(Mirn150)Rsky} mice were crossed to Tg(ACTFLPe)9205Dym mice to excise the frt-flanked IRES-EGFP cassette, producing mice slightly over-expressing Mirn150 (from the CAG promoter) in thymocytes. It is also possible to generate this allele from allele Gt(ROSA)26Sor^{tm14.1(Mirn150)Rsky}. (J:127234)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

ES cell line = R19 (ARF1 R19 ES cells from Artemis Pharmaceuticals, Germany).

References

• Original: J:127234 Xiao C, et al., MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb. Cell. 2007 Oct 5;131(1):146-59
• All: 3 reference(s)