Gcsam^tm1.1Rsky
Targeted Allele Detail

Summary

• Symbol: Gcsam^tm1.1Rsky
• Name: germinal center associated, signaling and motility; targeted mutation 1.1, Klaus Rajewsky
• MGI ID: MGI:3763735
• Synonyms: M17^-
• Gene: Gcsam Location: Chr16:45430803-45443230 bp, + strand Genetic Position: Chr16, 29.72 cM, cytoband B5
• Alliance: Gcsam^tm1.1Rsky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:125784
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 4.5 kb region containing exons 4 and 5 of the gene were flanked by loxP sites. A loxP flanked neo cassette was place upstream of exon 4, and a frt-flanked reporter cassette consisting of a splice acceptor site, an IRES domain, and a GFP gene was placed down stream of exon 5. Chimeric mice were crossed with a Cre-expressing transgenic, removing the neo cassette and the two coding exons. Inactivation of the gene was confirmed by RT-PCR on RNA from germinal center B-cells. No GFP expression was detected, possibly due to unstable RNA. (J:125784)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gcsam Mutation: 12 strains or lines available

References

• Original: J:125784 Schenten D, et al., M17, a gene specific for germinal center (GC) B cells and a prognostic marker for GC B-cell lymphomas, is dispensable for the GC reaction in mice. Blood. 2006 Jun 15;107(12):4849-56
• All: 1 reference(s)