Fn1^tm4Hyn
Targeted Allele Detail

Summary

• Symbol: Fn1^tm4Hyn
• Name: fibronectin 1; targeted mutation 4, Richard Hynes
• MGI ID: MGI:3764511
• Synonyms: EIIIA^-EIIIB^-, EIIIA/EIIIB-double-null
• Gene: Fn1 Location: Chr1:71624679-71692359 bp, - strand Genetic Position: Chr1, 36.05 cM, cytoband C1-C5
• Alliance: Fn1^tm4Hyn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:126737
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: ES cells from homozygous Fn1^tm1Ksek male mouse in which exon EDB (EIIIB) was deleted and replaced with a singe loxp site was isolated and targeted with the same construct used in Fn1^tm1Bwg. Following homologous recombination and transient cre-mediated recombination, a single loxp site was left in place of the exon encoding the EIIIA domain. Northern and Western blot confirmed the absence of EIII1 and EIIIB mRNA and protein and that deletion of both of those exons did not compromise the expression of FN mRNA and protein, nor the deposition of FN into the matrix. (J:126737)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Fn1 Mutation: 130 strains or lines available

Notes

ES cells were derived from male homozygous Fn1^tm1Ksek mouse of a mixed 129P2/OlaHsd and C57BL/6J background strain.

References

• Original: J:126737 Astrof S, et al., Multiple cardiovascular defects caused by the absence of alternatively spliced segments of fibronectin. Dev Biol. 2007 Nov 1;311(1):11-24
• All: 3 reference(s)