Gt(ROSA)26Sor^{tm2(cre/ERT2)Brn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(cre/ERT2)Brn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Anton Berns
• MGI ID: MGI:3764519
• Synonyms: ERT2, Gt(ROSA)26Sor^{tm2(cre/Esr1)Brn}, Rosa26CreERT2, ROSA26-CreERT2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(cre/ERT2)Brn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127226
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(cre/ERT2)Brn} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gt(ROSA)26Sor (MGI:104735)

• Mutation details: The endogenous locus was modified by insertion into the first intron of a construct containing the rabbit beta-globin 3' splice site followed by the cre/ERT2 fusion gene, which encodes Cre recombinase joined to a mutant hormone-binding domain of the human estrogen receptor that is activated by 4-hydroxytamoxifen (4-OHT), but not by the natural ER ligand 17-beta-estradiol. The fusion gene is followed by the rabbit beta-globin polyadenylation signal and a PGK-puro (puromycin resistance) cassette. Western blot analysis confirmed expression of the cre/ERT2 fusion product. (J:127226)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gt(ROSA)26Sor (mouse) Summary of all recombinase alleles driven by Gt(ROSA)26Sor. Comparative matrix view of recombinase activities Alliance mouse Gt(ROSA)26Sor gene page

Disease models

Expression

In Mice Carrying this Mutation:

6 assay results

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm2(cre/ERT2)Brn}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 994 strains or lines available

References

• Original: J:127226 Hameyer D, et al., Toxicity of ligand-dependent Cre recombinases and generation of a conditional Cre deleter mouse allowing mosaic recombination in peripheral tissues. Physiol Genomics. 2007 Sep 19;31(1):32-41
• All: 104 reference(s)