Itgb7^tm1Mshi
Targeted Allele Detail

Summary

• Symbol: Itgb7^tm1Mshi
• Name: integrin beta 7; targeted mutation 1, Motomu Shimaoka
• MGI ID: MGI:3764672
• Synonyms: Itgb7^D146A
• Gene: Itgb7 Location: Chr15:102124430-102140379 bp, - strand Genetic Position: Chr15, 57.39 cM
• Alliance: Itgb7^tm1Mshi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127413
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A vector consisting of a cre recombinase and a neomycin resistant gene under the control of the sperm-specific ACE promoter was used for targeting exon 4. In exon 4, the sequence encoding amino acid 146 was changed from an aspartate to an alanine (D146A). This mutation was predicted to keep the integrin in a constitutively adhesive state. The cre-recombinase and the neomycin resistance gene were excised in chimeras during spermatogenesis, leaving behind one loxP site. The mutation was confirmed by genomic sequencing. (J:127413)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Itgb7 Mutation: 72 strains or lines available

References

• Original: J:127413 Park EJ, et al., Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut. J Clin Invest. 2007 Sep;117(9):2526-38
• All: 2 reference(s)