Epm2a^{Tg(TcraK,TcrbK)TG-BFlv}
Transgenic Allele Detail

Summary

• Symbol: Epm2a^{Tg(TcraK,TcrbK)TG-BFlv}
• Name: epilepsy, progressive myoclonic epilepsy, type 2 gene alpha; transgene insertion TG-B, Richard A Flavell
• MGI ID: MGI:3764832
• Synonyms: H2-Kk, TG-B, Tg(TcraK,TcrbK)TG-BFlv
• Gene: Epm2a Location: Chr10:11219148-11335388 bp, + strand Genetic Position: Chr10, 3.66 cM, cytoband A
• Alliance: Epm2a^{Tg(TcraK,TcrbK)TG-BFlv} page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA/J)F2

Transgene description

• Transgene Type: Transgenic (Hypomorph, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The transgenic mice express rearranged Valpha and Vbeta genes from a CTL clone that recognized the SV40 large T antigen (SV-T). The V and J regions of a transgene construct (made in J:107523) were replaced with the V and J regions of the clone K T cell receptor. The synthetic alpha and beta constructs were coinjected into 1 day embryos. Three lines were obtained and line TG-B was further characterized. The transgene in line TG-B inserted into intron 1. Western blot analysis confirmed reduced protein expression. (J:107545, J:112696)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Epm2a Mutation: 28 strains or lines available

References

• Original: J:107545 Geiger T, et al., T-cell responsiveness to an oncogenic peripheral protein and spontaneous autoimmunity in transgenic mice. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2985-9
• All: 9 reference(s)