Trpc1^tm1Lbi
Targeted Allele Detail

Summary

• Symbol: Trpc1^tm1Lbi
• Name: transient receptor potential cation channel, subfamily C, member 1; targeted mutation 1, Lutz Birnbaumer
• MGI ID: MGI:3764882
• Gene: Trpc1 Location: Chr9:95587135-95632428 bp, - strand Genetic Position: Chr9, 50.2 cM, cytoband E4
• Alliance: Trpc1^tm1Lbi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127788
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An EcoRI site in exon 8 was converted to a stop codon and a PGK-neo selection cassette replaced the terminal part of exon 8 along with intron 8. This resulted in loss of the coding region for amino acids 584 - 670 encoding transmembrane segments 4 and 5 and a small part of the putative pore domain. RT-PCR analysis detected a shorter transcript in which all of exon 8 was skipped resulting in a frameshift and stop codon at position 2 of exon 9. Western blot analysis confirmed absence of expression in the submandibular glands of homozygous mice. (J:127118, J:127788)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trpc1 Mutation: 41 strains or lines available

References

• Original: J:127788 Dietrich A, et al., Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1. Pflugers Arch. 2007 Dec;455(3):465-77
• All: 59 reference(s)