Kdm3a^{Gt(YHA186)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Kdm3a^{Gt(YHA186)Byg}
• Name: lysine (K)-specific demethylase 3A; gene trap YHA186, BayGenomics
• MGI ID: MGI:3764891
• Synonyms: Jhdm2a^G
• Gene: Kdm3a Location: Chr6:71565954-71609963 bp, - strand Genetic Position: Chr6, 32.18 cM
• Alliance: Kdm3a^{Gt(YHA186)Byg} page

Mutation origin

• Mutant Cell Line: YHA186
• Germline Transmission: Earliest citation of germline transmission: J:127121
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: A beta-galactosidase/neomycin (beta-geo)cassette was inserted into intron 10 (also intron 7 of splice variant 2). RT-PCR analysis confirmed the expression of a fusion transcript (exons 1-10 fused to beta-geo) and a low level of wild-type RNA expression (about 10% that of wild-type controls) in homozygous mutants. (J:127121)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  YHA186	CZ490855 ; (MGI Seq Detail)	5' RACE	Chr6:71588551-71588785 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  55 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm3a Mutation: 81 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:127121 Okada Y, et al., Histone demethylase JHDM2A is critical for Tnp1 and Prm1 transcription and spermatogenesis. Nature. 2007 Nov 1;450(7166):119-23
• All: 4 reference(s)