Ep400^tm1Fuku
Targeted Allele Detail

Summary

• Symbol: Ep400^tm1Fuku
• Name: E1A binding protein p400; targeted mutation 1, Rikiro Fukunaga
• MGI ID: MGI:3764913
• Synonyms: mDom^deltaN
• Gene: Ep400 Location: Chr5:110812239-110918583 bp, - strand Genetic Position: Chr5, 53.69 cM, cytoband F
• Alliance: Ep400^tm1Fuku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127131
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon and intron 2 were replaced with a neo cassette. The absence of transcript was confirmed by whole mount in situ hybridization using E8.5 embryos. An aberrant transcript lacking the 471 N-terminal amino acids is produced from an initiation site in exon 3. The lack of the full-length protein product and presence of a shorter peptide lacking the N-terminus was confirmed by western blot analysis on embryonic fibroblasts derived from E8.5 embryos. (J:127131)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ep400 Mutation: 142 strains or lines available

References

• Original: J:127131 Ueda T, et al., Critical role of the p400/mDomino chromatin-remodeling ATPase in embryonic hematopoiesis. Genes Cells. 2007 May;12(5):581-92
• All: 2 reference(s)