Tg(GUSB)4Sly
Transgene Detail

Summary

• Symbol: Tg(GUSB)4Sly
• Name: transgene insertion 4, William S Sly
• MGI ID: MGI:3764916
• Synonyms: TgGUSB
• Transgene: Tg(GUSB)4Sly Location: unknown
• Alliance: Tg(GUSB)4Sly page

Lysosomal storage disease is evident in Gusb^mps/Gusb^mps mice and is rescued by Tg(GUSB)4Sly/Tg(GUSB)4Sly expression

Show the 1 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(GUSB)4Sly expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GUSB (2990)

  Gusb	(MGI:95872)

• Mutation details: The entire human beta-glucuronidase gene with 1.6kb of 5'-flanking sequence and 3.8kb of 3'-flanking sequence was microinjected into C57BL/6J zygotes. Founder line 4 was subsequently propagated. Mice that are homozygous for this transgene and the Gusb^mps allele have approximately 20-fold higher beta-glucuronidase enzyme activity than wildtype controls. Distribution of human enzyme activity throughout various tissues mimics the endogenous mouse enzyme activity pattern. (J:21256)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:21256 Kyle JW, et al., Correction of murine mucopolysaccharidosis VII by a human beta-glucuronidase transgene. Proc Natl Acad Sci U S A. 1990 May;87(10):3914-8
• All: 1 reference(s)