Apc^tm1.1Tno
Targeted Allele Detail

Summary

• Symbol: Apc^tm1.1Tno
• Name: APC, WNT signaling pathway regulator; targeted mutation 1.1, Tetsuo Noda
• MGI ID: MGI:3764940
• Synonyms: Apc^580D, Apc^580delta
• Gene: Apc Location: Chr18:34353977-34455605 bp, + strand Genetic Position: Chr18, 18.53 cM
• Alliance: Apc^tm1.1Tno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127452
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: One loxP site was inserted into intron 13 and the other, with a PGK-neo cassette, into intron 14. Exon 14 was excised by Cre mediated recombination, resulting in a nonsense frame-shift mutation. Excision of the exon was confirmed by RT-PCR and sequencing analysis of mRNA isolated from the large intestine. Western blot detected the truncated product (70 kDa). (J:127452, J:156864)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apc Mutation: 158 strains or lines available

References

• Original: J:127452 Shibata H, et al., Alpha-catenin is essential in intestinal adenoma formation. Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18199-204
• All: 3 reference(s)