Grid2^tm1Mmsh
Targeted Allele Detail

Summary

• Symbol: Grid2^tm1Mmsh
• Name: glutamate receptor, ionotropic, delta 2; targeted mutation 1, Masayoshi Mishina
• MGI ID: MGI:3765001
• Synonyms: GluRdelta2deltaT
• Gene: Grid2 Location: Chr6:63232860-64681307 bp, + strand Genetic Position: Chr6, 29.77 cM
• Alliance: Grid2^tm1Mmsh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127449
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 21 nucleotide deletion encoding 7 C-terminal amino acids (the T site) was created in exon 16. An frt-flanked fragment containing a PKG-neo and a Tn5 promoter-driven chloramphenicol-acetyltransferase was inserted 1.6 kb downstream of deletion in exon 16. Western blot analysis confirmed expression is 76.3+/-3.0% of wild-type. (J:127449)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Grid2 Mutation: 85 strains or lines available

References

• Original: J:127449 Uemura T, et al., Regulation of long-term depression and climbing fiber territory by glutamate receptor delta2 at parallel fiber synapses through its C-terminal domain in cerebellar Purkinje cells. J Neurosci. 2007 Oct 31;27(44):12096-108
• All: 3 reference(s)