Fasn^tm1.1Sem
Targeted Allele Detail

Summary

• Symbol: Fasn^tm1.1Sem
• Name: fatty acid synthase; targeted mutation 1.1, Clay F Semenkovich
• MGI ID: MGI:3765071
• Synonyms: FAS-deficient
• Gene: Fasn Location: Chr11:120696672-120715373 bp, - strand Genetic Position: Chr11, 84.56 cM
• Alliance: Fasn^tm1.1Sem page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127793
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Mice carrying the Fasn^tm1Sem allele were crossed with Cre transgenic mice to remove exons 4-8 of the gene. Germline deletion of the exons was verified by PCR, and gene inactivation was demonstrated by a 50% reduction in enzyme activity in liver lysates from heterozygote. (J:127793)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fasn Mutation: 89 strains or lines available

References

• Original: J:127793 Chakravarthy MV, et al., 'New' hepatic fat activates PPARalpha to maintain glucose, lipid, and cholesterol homeostasis. Cell Metab. 2005 May;1(5):309-22
• All: 3 reference(s)