Cd4^tm1Doi
Targeted Allele Detail

Summary

• Symbol: Cd4^tm1Doi
• Name: CD4 antigen; targeted mutation 1, Christophe Benoist and Diane Mathis
• MGI ID: MGI:3765235
• Synonyms: LAG⁰CD4⁰
• Gene: Cd4 Location: Chr6:124841655-124865184 bp, - strand Genetic Position: Chr6, 59.17 cM
• Alliance: Cd4^tm1Doi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33272
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: ES cells containing a previously targeted Lag3 allele (Lag3^tm1Doi) were targeted again with a vector replacing part of exon 5 of the Cd4 gene with a hygromycin selection cassette. Detection of doubly mutant embryos was determined by PCR and Southern blotting. (J:33272)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd4 Mutation: 85 strains or lines available

References

• Original: J:33272 Miyazaki T, et al., LAG-3 is not responsible for selecting T helper cells in CD4-deficient mice. Int Immunol. 1996 May;8(5):725-9
• All: 1 reference(s)