Cd4^tm1Doi
Targeted Allele Detail

Summary

• Symbol: Cd4^tm1Doi
• Name: CD4 antigen; targeted mutation 1, Christophe Benoist and Diane Mathis
• MGI ID: MGI:3765235
• Synonyms: LAG⁰CD4⁰
• Gene: Cd4 Location: Chr6:124841655-124865184 bp, - strand Genetic Position: Chr6, 59.17 cM
• Alliance: Cd4^tm1Doi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33272
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: ES cells containing a previously targeted Lag3 allele (Lag3^tm1Doi) were targeted again with a vector replacing part of exon 5 of the Cd4 gene with a hygromycin selection cassette. Detection of doubly mutant embryos was determined by PCR and Southern blotting. (J:33272)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cx1	Cd4^tm1Doi/Cd4^tm1Doi Lag3^tm1Doi/Lag3^tm1Doi	involves: 129 * C57BL/6
cx2	Cd4^tm1Doi/Cd4^tm1Litt Lag3^tm1Doi/Lag3^+	involves: 129 * C57BL/6

Phenotypes:

Affected Systems	cx1	cx2
show or hide all annotated terms
hematopoietic system	√	√
increased double-negative T cell number	√	√
abnormal CD4-positive, alpha-beta T cell physiology	√	√
immune system	√	√
increased double-negative T cell number	√	√
abnormal CD4-positive, alpha-beta T cell physiology	√	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd4 Mutation: 84 strains or lines available

References

• Original: J:33272 Miyazaki T, et al., LAG-3 is not responsible for selecting T helper cells in CD4-deficient mice. Int Immunol. 1996 May;8(5):725-9
• All: 1 reference(s)