Plxnb1^tm1Matl
Targeted Allele Detail

Summary

• Symbol: Plxnb1^tm1Matl
• Name: plexin B1; targeted mutation 1, Marc Tessier-Lavigne
• MGI ID: MGI:3765917
• Synonyms: Plxnb1-, Plxnb1-lacZ
• Gene: Plxnb1 Location: Chr9:108924457-108948985 bp, + strand Genetic Position: Chr9, 59.63 cM
• Alliance: Plxnb1^tm1Matl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139221
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Targeted mutagenesis using a promoterless gene trap vector ("targeted trapping") was used to insert a 9.4-kb placental alkaline phosphatase (PLAP) secretory cassette into intron 21. This results in transcription of a fusion mRNA joining upstream exons of the endogenous gene to the elements of the cassette, comprising a splice acceptor and a transmembrane domain coding sequence followed by the betageo (lacZ/neomycin resistance fusion) cDNA, an internal ribosomal entry site (IRES), and the human PLAP sequence with a polyadenylation signal. Two proteins are translated from this bicistronic transcript: a PLXNB1/Betageo fusion protein that is sequestered in an intracellular compartment of the neuronal cell body; and PLAP, which is expressed on the axonal surface. (Roland Freidel, data submission). Western blot of E11.5 whole embryo lysates confirmed the absence of protein in homozygous mutants. (J:228347)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Plxnb1 Mutation: 108 strains or lines available

References

• Original: J:121225 Friedel RH, et al., Gene targeting using a promoterless gene trap vector ('targeted trapping') is an efficient method to mutate a large fraction of genes. Proc Natl Acad Sci U S A. 2005 Sep 13;102(37):13188-93
• All: 8 reference(s)