Ltbp1^tm2Veto
Targeted Allele Detail

Summary

• Symbol: Ltbp1^tm2Veto
• Name: latent transforming growth factor beta binding protein 1; targeted mutation 2, Vesna Todorovic
• MGI ID: MGI:3765968
• Synonyms: Ltbp1L^-
• Gene: Ltbp1 Location: Chr17:75312563-75699507 bp, + strand Genetic Position: Chr17, 46.24 cM, cytoband E3
• Alliance: Ltbp1^tm2Veto page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128012
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 7.8 kb fragment from codon 165 in exon 2 through exon 2 and its downstream intron were deleted and replaced with a lacZ gene and neo cassette using Velocigene technology. The neo cassette was removed by cre-mediated recombination. The absence of protein was confirmed by western blot analysis on protein extracts from E14.5 hearts. (J:128012)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ltbp1 Mutation: 115 strains or lines available

Notes

Ltbp1^tm1Veto and Ltbp1^tm2Veto mice exhibit identical defects.

References

• Original: J:128012 Todorovic V, et al., Long form of latent TGF-beta binding protein 1 (Ltbp1L) is essential for cardiac outflow tract septation and remodeling. Development. 2007 Oct;134(20):3723-32
• All: 3 reference(s)