Akap5^tm1Gsm
Targeted Allele Detail

Summary

• Symbol: Akap5^tm1Gsm
• Name: A kinase anchor protein 5; targeted mutation 1, G Stanley Mcknight
• MGI ID: MGI:3765995
• Synonyms: AKAP150delta36, AKAP150 delta A, D36
• Gene: Akap5 Location: Chr12:76371665-76380927 bp, + strand Genetic Position: Chr12, 33.73 cM
• Alliance: Akap5^tm1Gsm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127739
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A stop codon (TAG) replaced the codon for Leu710 (TTA) (L710X), truncating the last 36 residues that are important for binding to protein kinase A (PKA). A loxP-flanked neomycin resistance cassette was also inserted downstream of the lone coding exon. After germ line transmission, the neomycin resistance cassette was removed by crossing with cre-transgenic mice. Western blots for PKA subunits from postsynaptic densities of the forebrain indicated reduced levels of protein, confirming mutation of the targeted gene. (J:127739, J:160134)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Akap5 Mutation: 27 strains or lines available

References

• Original: J:127739 Lu Y, et al., Age-dependent requirement of AKAP150-anchored PKA and GluR2-lacking AMPA receptors in LTP. EMBO J. 2007 Nov 28;26(23):4879-90
• All: 8 reference(s)