Tg(Tnf)6074Gkl
Transgene Detail

Summary

• Symbol: Tg(Tnf)6074Gkl
• Name: transgene insertion 6074, George Kollias
• MGI ID: MGI:3766101
• Synonyms: Tg6074
• Transgene: Tg(Tnf)6074Gkl Location: unknown
• Alliance: Tg(Tnf)6074Gkl page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tnf)6074Gkl expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tnf (MGI:104798)

• Mutation details: These mice express a construct which contains the promoter and entire coding region of the murine TNF-alpha gene. A 0.77-kb EcoRI-Pst I/Sal I fragment, containing the 3'-untranslated region and polyadenylylation site of the human beta-globin gene was ligated to the 3' end of the murine gene. The transgene is expressed specifically in neuron cell bodies of the CNS and is detected in all areas tested including the cerebral cortex, cerebellum, diencephalon, brain stem, and spinal cord. Expression is not detected in astrocyte cultures, activated peritoneal macrophages or activated splenocytes. Expression is first detectable at low levels in P6 mice and increases greatly in intensity until P15 after which expression remains at high levels. (J:111326)

Disease models

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

model for multiple sclerosis (J:89603)

References

• Original: J:111326 Probert L, et al., Spontaneous inflammatory demyelinating disease in transgenic mice showing central nervous system-specific expression of tumor necrosis factor alpha. Proc Natl Acad Sci U S A. 1995 Nov 21;92(24):11294-8
• All: 12 reference(s)