Vac14^{Gt(RRP155)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Vac14^{Gt(RRP155)Byg}
• Name: Vac14 homolog (S. cerevisiae); gene trap RRP155, BayGenomics
• MGI ID: MGI:3766320
• Synonyms: Vac14^beta-geo
• Gene: Vac14 Location: Chr8:111345217-111447030 bp, + strand Genetic Position: Chr8, 57.59 cM
• Alliance: Vac14^{Gt(RRP155)Byg} page

Neural tissue lesions in Vac14^{Gt(RRP155)Byg}/Vac14^{Gt(RRP155)Byg} mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: RRP155
• Germline Transmission: Earliest citation of germline transmission: J:127106
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A beta-geo gene trap vector inserted into intron 1. Western blot analysis confirmed the absence of protein expression in extracts from primary fibroblasts. In primary fibroblasts levels of phophatidylinositol 3,5-biphosphate and phophatidylinositol 5-phosphate are significantly reduced while levels of phophatidylinositol 3-phosphate are elevated. (J:127106)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRP155	CG671760 ; (MGI Seq Detail)	5' RACE	Chr8:111345534-111345624 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  6 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

20 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vac14 Mutation: 43 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:127106 Zhang Y, et al., Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice. Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17518-23
• All: 7 reference(s)