Tg(Thy1-EGFP)MJrs
Transgene Detail

Summary

• Symbol: Tg(Thy1-EGFP)MJrs
• Name: transgene insertion M, Joshua R Sanes
• MGI ID: MGI:3766828
• Synonyms: GFP line-M, GFP-M, Thy1-GFP line M, Thy1-GFP/M, Thy1-GFPM, Thy1::GFP-M
• Transgene: Tg(Thy1-EGFP)MJrs Location: unknown
• Alliance: Tg(Thy1-EGFP)MJrs page

Transgene origin

• Strain of Origin: (C57BL/6J x CBA)F1

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The Thy1-GFP transgene was designed with a modified regulatory region of the "murine thy1.2 gene" (extending from the promoter to the intron following exon 4, excluding exon 3 and its flanking introns) upstream of an enhanced green fluorescent protein (EGFP) sequence. Line M was subsequently established and analyzed. Expression of EGFP is detected primarily in mossy fibers in internal granule layer of the cerebellum (10-80% incidence). Less than 10% of all motor axons, retinal ganglion cells, lumbar dorsal root ganglion, and cortex express EGFP, with no reported expression in other tested neuronal tissues. (J:93699)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:93699 Feng G, et al., Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron. 2000 Oct;28(1):41-51
• All: 237 reference(s)