Gt(ROSA)26Sor^{tm3(phiC31*)Sor}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(phiC31*)Sor}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Philippe Soriano
• MGI ID: MGI:3766932
• Synonyms: R26phiC31o
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(phiC31*)Sor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128069
• Parent Cell Line: AK7.1 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3(phiC31*)Sor} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gt(ROSA)26Sor (MGI:104735)

• Mutation details: A targeting construct with the SA-PhiC31opA-PGKneopA cassette, which contains the mouse codon-optimized site-specific recombinase PhiC31o sequence (with a C-terminal SV40 nuclear localization signal) driven by the phosphoglycerate kinase 1 (PGK) promoter, and bovine growth hormone polyadenylation (bpA) sequence was inserted into the Gt(ROSA)26Sor locus. Flp-mediated recombination removes the neo cassette. PhiC31o-mediated recombination in mice carrying containing a sequence of interest flanked by attB and attP sites results in tissue-specific deletion of the targeted sequence. (J:128069)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Gt(ROSA)26Sor (mouse) Summary of all recombinase alleles driven by Gt(ROSA)26Sor. Comparative matrix view of recombinase activities Alliance mouse Gt(ROSA)26Sor gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:128069 Raymond CS, et al., High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS One. 2007;2(1):e162
• All: 20 reference(s)