Gt(ROSA)26Sor^{tm4(attB/attP)Sor}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(attB/attP)Sor}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Philippe Soriano
• MGI ID: MGI:3766933
• Synonyms: R26attR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(attB/attP)Sor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128069
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A targeting construct with the SA-attB-PGKneo 4xpA-attP-lacZ-pA cassette, in which the STOP fragment is flanked by attB and attP sites (phi31 recognition sites), was inserted into the Gt(ROSA)26Sor. Expression of the lacZ gene is blocked by a STOP fragment (flanked by attB and attP sites) placed between the lacZ sequence and the Gt(ROSA)26Sor promoter. PhiC31o-mediated recombination will excise the STOP and allow lacZ transcription. (J:128069)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:128069 Raymond CS, et al., High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS One. 2007;2(1):e162
• All: 3 reference(s)