Tg(NPHS2-rtTA,tetO-cre)1Holt
Transgene Detail

Summary

• Symbol: Tg(NPHS2-rtTA,tetO-cre)1Holt
• Name: transgene insertion 1, Harry Holthofer
• MGI ID: MGI:3767326
• Synonyms: JRC-CRE
• Transgene: Tg(NPHS2-rtTA,tetO-cre)1Holt Location: unknown
• Alliance: Tg(NPHS2-rtTA,tetO-cre)1Holt page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(NPHS2-rtTA,tetO-cre)1Holt expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  NPHS2 (7827)

  Nphs2

  (MGI:2157018)

• Mutation details: A rtTA gene modified to respond to less doxycycline was placed under the control of the human NPHS2 gene, which is only expressed in the podocytes of the kidney. In the same vector, a modified CMV promoter with a rtTA responsive element was used to drive the expression of cre recombinase. Sixteen founder mice were created, and one was selected for further breeding based on the doxycycline-induced expression of cre recombinase that was only detected in kidney podocyte cells. (J:128251)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	NPHS2 (human) Summary of all recombinase alleles driven by NPHS2. Comparative matrix view of recombinase activities Alliance human NPHS2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Of 16 founder lines created, one was chosen for further breeding and study.

References

• Original: J:128251 Juhila J, et al., Podocyte cell-specific expression of doxycycline inducible Cre recombinase in mice. J Am Soc Nephrol. 2006 Mar;17(3):648-54
• All: 2 reference(s)