Rapgef4^tm1.1Sse
Targeted Allele Detail

Summary

• Symbol: Rapgef4^tm1.1Sse
• Name: Rap guanine nucleotide exchange factor (GEF) 4; targeted mutation 1.1, Susumu Seino
• MGI ID: MGI:3767602
• Synonyms: Epac2^ko
• Gene: Rapgef4 Location: Chr2:71811584-72087818 bp, + strand Genetic Position: Chr2, 42.9 cM, cytoband C3
• Alliance: Rapgef4^tm1.1Sse page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128493
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into exon 1 and a floxed neo cassette was inserted downstream of exon 1. Cre-mediated recombination was used to delete a portion of exon 1. The absence of protein product in pancreatic islet cells was confirmed by RT-PCR analysis. (J:128493)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rapgef4 Mutation: 66 strains or lines available

References

• Original: J:128493 Shibasaki T, et al., Essential role of Epac2/Rap1 signaling in regulation of insulin granule dynamics by cAMP. Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19333-8
• All: 14 reference(s)