Tg(Tek-Jam3)1Maal
Transgene Detail

Summary

• Symbol: Tg(Tek-Jam3)1Maal
• Name: transgene insertion 1, Michel Aurrand-Lions
• MGI ID: MGI:3767832
• Synonyms: pHHNS-JAM-C, Tg(Tie2-Jam-C)1Maal
• Transgene: Tg(Tek-Jam3)1Maal Location: unknown
• Alliance: Tg(Tek-Jam3)1Maal page

Transgene origin

• Strain of Origin: CBA x C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tek-Jam3)1Maal expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Jam3 (MGI:1933825)

• Mutation details: The transgene contains the coding sequence, 3' untranslated region and polyadenylation signal of mouse junction adhesion molecule 3 (Jam3) inserted near the 3' end of the first exon of the mouse endothelial-specific receptor tyrosine kinase gene (Tek) in a genomic DNA fragment containing 2.1 kb of upstream DNA containing Tek promoter sequences, most of exon 1 and, following the inserted Jam3 sequence, ~10 kb of the first half of intron 1, which contains endothelial cell-specific enhancer elements.

  Western blot analysis of total lung lysates and of immunoprecipitates of lung lysates demonstrate elevated expression of JAM3 in lungs of transgenic mice versus control littermates. Flow cytometric analysis of dissociated lung tissue cells stained for JAM3, the endothelial-cell marker VE-cadherin and the hematopoietic cell marker CD45 show 3-fold enhanced expression of JAM3 specifically on endothelial cells. (J:99020)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:99020 Aurrand-Lions M, et al., Junctional adhesion molecule-C regulates the early influx of leukocytes into tissues during inflammation. J Immunol. 2005 May 15;174(10):6406-15
• All: 6 reference(s)