Gpr83^tm1.1Ayr
Targeted Allele Detail

Summary

• Symbol: Gpr83^tm1.1Ayr
• Name: G protein-coupled receptor 83; targeted mutation 1.1, Alexander Y Rudensky
• MGI ID: MGI:3768257
• Synonyms: GPR83^-
• Gene: Gpr83 Location: Chr9:14771506-14782085 bp, + strand Genetic Position: Chr9, 4.4 cM, cytoband A2-A3
• Alliance: Gpr83^tm1.1Ayr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:127791
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP element was inserted 347 bp upstream of exon 3, and a cassette containing a loxP site and a FRT-flanked neomycin cassette was inserted 703 bp downstream of exon 4. Chimeric mice were first mated to FLP deleter mice to remove the neomycin cassette, and subsequently to Cre-transgenic mice to remove the floxed alleles. Gene deletion was confirmed by lack of expression in regulatory T cells as determined by quantitative RT-PCR. (J:127791)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gpr83 Mutation: 26 strains or lines available

References

• Original: J:127791 Lu LF, et al., G protein-coupled receptor 83 is dispensable for the development and function of regulatory T cells. Mol Cell Biol. 2007 Dec;27(23):8065-72
• All: 3 reference(s)