Scn1b^tm2Isom
Targeted Allele Detail

Summary

• Symbol: Scn1b^tm2Isom
• Name: sodium channel, voltage-gated, type I, beta; targeted mutation 2, Lori L Isom
• MGI ID: MGI:3768513
• Synonyms: Scn1b^flox, Scn1b^neo
• Gene: Scn1b Location: Chr7:30815949-30826428 bp, - strand Genetic Position: Chr7, 19.3 cM
• Alliance: Scn1b^tm2Isom page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128713
• Parent Cell Line: Pat5 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites were inserted 190 bp upstream and 220 bp downstream of exon 2. A FRT-flanked neomycin cassette was also inserted further downstream of exon 2. Germline transmission was confirmed by genomic PCR. The presence of the loxP sites or the neocassette was determined not to alter gene expression. (J:128713)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scn1b Mutation: 8 strains or lines available

References

• Original: J:128713 Chen C, et al., Floxed allele for conditional inactivation of the voltage-gated sodium channel beta1 subunit Scn1b. Genesis. 2007 Sep;45(9):547-53
• All: 1 reference(s)