Tgfb3^tm2(Tgfb1)Vk
Targeted Allele Detail

Summary

• Symbol: Tgfb3^tm2(Tgfb1)Vk
• Name: transforming growth factor, beta 3; targeted mutation 2, Vesa Kaartinen
• MGI ID: MGI:3768675
• Synonyms: Tgfb1-KI
• Gene: Tgfb3 Location: Chr12:86103519-86125815 bp, - strand Genetic Position: Chr12, 40.09 cM
• Alliance: Tgfb3^tm2(Tgfb1)Vk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:128938
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A vector containing Tgfb1 cDNA followed by a pgk polyA sequence and a loxP flanked neomycin resistance cassette was inserted into the ATG start site of the targeted gene. Quantitative RT-PCR of RNA isolated from the tips of the palatal shelves demonstrates high Tgfb1 expression but no Tgfb3 expression in homozygotes. (J:128938)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tgfb3 Mutation: 35 strains or lines available

References

• Original: J:128938 Yang LT, et al., Tgfb1 expressed in the Tgfb3 locus partially rescues the cleft palate phenotype of Tgfb3 null mutants. Dev Biol. 2007 Dec 1;312(1):384-95
• All: 1 reference(s)