Pold1^tm2Loeb
Targeted Allele Detail

Summary

• Symbol: Pold1^tm2Loeb
• Name: polymerase (DNA directed), delta 1, catalytic subunit; targeted mutation 2, Lawrence A Loeb
• MGI ID: MGI:3769034
• Synonyms: Pold1^L604K
• Gene: Pold1 Location: Chr7:44182168-44198239 bp, - strand Genetic Position: Chr7, 28.83 cM
• Alliance: Pold1^tm2Loeb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:129046
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A nucleotide substitution in exon 15 resulted in the amino acid substitution of lysine for leucine at position 604 (L604K). This amino acid is located in a highly conserved motif A in the active site of the polymerase domain. An neo cassette was also inserted into intron 11. Expression was confirmed by western blot analysis on extracts from the liver. (J:129046)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pold1^tm2Loeb

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pold1 Mutation: 63 strains or lines available

References

• Original: J:129046 Venkatesan RN, et al., Mutation at the polymerase active site of mouse DNA polymerase delta increases genomic instability and accelerates tumorigenesis. Mol Cell Biol. 2007 Nov;27(21):7669-82
• All: 1 reference(s)