Sirt5^tm1Fwa
Targeted Allele Detail

Summary

• Symbol: Sirt5^tm1Fwa
• Name: sirtuin 5; targeted mutation 1, Frederick W Alt
• MGI ID: MGI:3769236
• Gene: Sirt5 Location: Chr13:43518972-43548679 bp, + strand Genetic Position: Chr13, 21.6 cM
• Alliance: Sirt5^tm1Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:129009
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 through 5 were replaced with a lacZ gene inserted in frame after the 23rd base pair of exon 2. The absence of protein product was confirmed by western blot analysis on liver extracts. (J:129009)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sirt5 Mutation: 29 strains or lines available

References

• Original: J:129009 Lombard DB, et al., Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation. Mol Cell Biol. 2007 Dec;27(24):8807-14
• All: 29 reference(s)