Hp^tm1Alev
Targeted Allele Detail

Summary

• Symbol: Hp^tm1Alev
• Name: haptoglobin; targeted mutation 1, Andrew P Levy
• MGI ID: MGI:3769352
• Synonyms: Hp2
• Gene: Hp Location: Chr8:110301760-110305804 bp, - strand Genetic Position: Chr8, 57.11 cM
• Alliance: Hp^tm1Alev page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:129061
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeted gene with its 5 coding exons has a high homology with a human gene that also has 5 coding exons. In humans, but not in mice, a second related gene exists where an in-frame duplication of exons 3 and 4 gives rise to a gene with 7 coding exons. To mimic this related gene in mice, murine coding sequence of exons 3 and 4 were placed upstream of exon 3 to create a "3-4-3" exon with no internal intronic sequence. The endogenous exon 3 was replaced with this 3-4-3 exon and a loxP-flanked neomycin selection cassette was inserted upstream. After crossing with Cre transgenic mice, the neomycin selection cassette was removed leaving some vector sequence within intron 2. After these events, the targeted locus has the following genomic structure: exon 1-intron 1-exon 2-intron 2 (with some vector sequence)-exon 3-exon 4-exon 3-intron 3-exon 4-intron 4-exon 5. Expression of the gene was confirmed by gel electrophoresis. (J:129061, J:134942)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hp Mutation: 13 strains or lines available

References

• Original: J:129061 Asleh R, et al., Haptoglobin genotype is a regulator of reverse cholesterol transport in diabetes in vitro and in vivo. Circ Res. 2006 Dec 8;99(12):1419-25
• All: 5 reference(s)