Actb^tm2Npa
Targeted Allele Detail

Summary

• Symbol: Actb^tm2Npa
• Name: actin, beta; targeted mutation 2, Novartis Pharma AG
• MGI ID: MGI:3769438
• Synonyms: FRT
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^tm2Npa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:129105
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: BALB/c

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, RMCE-ready, Reporter)
• Mutation: Insertion
• Mutation details: A vector was constructed where a recombinase-mediated cassette exchange (RMCE) site was placed downstream of a floxed stop cassette. The RMCE site consisted of a mutant FRT site, a promoterless EGFP reporter gene, a neomycin selection cassette and a wt FRT site. The vector targeted exon 2 of the beta-actin gene and disrupted its expression. Heterozygous animals had no reporter gene expression as assessed by fluorescent microscopy of brain, heart, liver and other organs. Upon removal of the stop codon by Cre recombinase, strong EGFP expression was observed. ES cells containing this allele allow for 1) RMCE into the b-actin locus and 2) initiating expression of the exchanged cassette by Cre-mediated excision of the stop codon. (J:129105)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 53 strains or lines available

References

• Original: J:129105 Jagle U, et al., Conditional transgene expression mediated by the mouse beta-actin locus. Genesis. 2007 Nov;45(11):659-66
• All: 2 reference(s)