Arhgef1^tm1Djle
Targeted Allele Detail

Summary

• Symbol: Arhgef1^tm1Djle
• Name: Rho guanine nucleotide exchange factor 1; targeted mutation 1, David J Lerner
• MGI ID: MGI:3769502
• Synonyms: Lsc^-
• Gene: Arhgef1 Location: Chr7:24602337-24626019 bp, + strand Genetic Position: Chr7, 13.52 cM
• Alliance: Arhgef1^tm1Djle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:129331
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 1370 nucleaotide upstream of the start codon through the first 13 exons was replaced with a neo cassette eliminating the first 420 amino acids of the protein. The absence of protein was confirmed by western blot analysis on splenocyte and neutrophil extracts. (J:129331)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arhgef1 Mutation: 73 strains or lines available

References

• Original: J:129331 Francis SA, et al., Rho GEF Lsc is required for normal polarization, migration, and adhesion of formyl-peptide-stimulated neutrophils. Blood. 2006 Feb 15;107(4):1627-35
• All: 5 reference(s)